Apparently Successful Cultivation of the Bacillus Leprae

Cultivation and Viability Determination of Mycobacterium leprae

Mycobacterium leprae, despite being recognized as a human pathogen over 140 years ago, remains uncultivable in microbiological culture media or in cell culture systems. Although it is now well established that M. leprae prefers cooler temperatures, slightly acidic microaerophilic conditions, and lipids rather than sugars as an energy source, the exact parameters for a defined axenic medium that...

The mouse foot-pad technique for cultivation of Mycobacterium leprae.

Although multiplication of Mycobacterium leprae in the foot pads of immune-competent mice is limited, and no leprosy-like lesions are produced in these animals, the mouse foot-pad system represents the first truly useful and reproducible animal model of M. leprae infection. Its employment has enabled research into basic questions with respect to the microbiology of M. leprae, and the epidemiolo...

The Cultivation of the Bacillus Lepræ

An Apparently Successful Case of Cardiac Massage

ago. Present confinement.?The membranes ruptured with the onset of labour and it was decided to apply forceps on account of primary uterine inertia which was contributed to by a cicatrical contraction in the vaginal canal about two inches above the vulva. Under chloroform ana;sthesia this was manually dilated and as forceps were being applied the patient suddenly stopped breathing. The forceps ...

Cultivation of Whipple bacillus: the irony and the ecstasy.